Almond Upton, 60, of Melrose, Florida pleaded not guilty to numerous charges including 1st degree murder, assault, reckless driving, and criminal mischief.
A grand jury had handed up an indictment against Upton last week.
Upton is accused of intentionally veering into state trooper Christopher Skinner, 42, who had pulled over a vehicle for speeding in the Town of Chenango.
More than ten members of the New York State Police and Skinner's family were in court Wednesday.
Major Donald Faughnan said it's important for police to be at the proceedings to show Skinner's family that it is not alone.
"It's just a support for the family and we'll continue to do that as this whole process moves forward and even after sentencing. We're there for the family. We're going to remain there," he said.
Trooper Skinner was part of a special construction zone traffic unit with the state police.
Broome County Court Judge Joe Cawley set a conference between himself, the district attorney and Upton's public defender for August 29 to look at the case.
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